| dc.contributor.author | El Abiad, Salma Abdu Allah | |
| dc.contributor.author | Elkilany, Atia Ramadan | |
| dc.date.accessioned | 2021-06-29T11:26:27Z | |
| dc.date.available | 2021-06-29T11:26:27Z | |
| dc.date.issued | 2016-01 | |
| dc.identifier.uri | http://dspace.elmergib.edu.ly/xmlui/handle/123456789/508 | |
| dc.description.abstract | The Swedish National Authority reported the presence of
elevated levels of acrylamide (ACR) in certain types of food
processed at high temperature. The present study was performed to
evaluate the toxicity of ACR in different tissues ofthe weaning
male and female rats after 14 and 28 days as well as two weeks of
ACR stopping effect. ACR induced inhibition in the activities of
the liver aminotransferases (ALT and AST)and alkaline
phosphatase (ALP). Brain acetylcholinesterase (AChE) activity
was significantly decreased in the male treated rats only. Stopping
of ACR could not resume the activities of the studied enzymes.
ACR induced a general decrease effect in glutathione reduced
(GSH) level in the different studied tissues of male and female
rats. Malondialdehyde (MDA) level significantly increased in liver
and brain of both male and female rats following administration of
ACR for 14 and 28 days.Acrylamide also showed significant
inhibition in the catalase (CAT)activityin the all studied tissues
following 14 and 28 days. The present study recommends
restriction of ACR exposure either occupationally or in food
containing product especially for children.Estimation of
enzymatic activities in liver: Liver aspartate aminotransferase (AST; EC 2. 6. 1. 1) And alanine aminotransferase (ALT; EC 2. 6.
1. 2) activities were assayed by the method of Reitman and Frankel
[32], while liver alkaline phosphatase (ALP ; EC 3. 1. 3. 1) activity
was assayed using the method of Belfield and Goldberg [33].
Brain acetylcholinesterase (AChE; EC 3. 1. 1. 7) activity was
estimated using acetythiocholine iodide as a substrate [34].
Estimation of oxidative stress markers: Lipid peroxidation in the
supernatants of different tissue organs was measured by the
formation of malondialdehyde (MDA) method [36]. The level of
total acid-soluble SH compound (glutathione GSH) in the different
tissues was determined according to Aykac et al. [37]. Superoxide
dismutase (SOD) was determined according toNishikimi[38]. | en_US |
| dc.language.iso | en | en_US |
| dc.publisher | Elmergib Uinversity | en_US |
| dc.subject | Acrylamide - Weaning rats- Aminotransferases – MDA – GSH – AChE. | en_US |
| dc.title | Oxidative stress as a risk factor of the acrylamide toxicity in the weaning male and female rats | en_US |
| dc.type | Other | en_US |
