Comparative Analysis of Bacterial Diversity in the Water Samples from Domestic wastewater through Metagenomics Technique

Abstract

Abstract In the recent years the development of genomics and introduced new techniques and greater possibilities in the study of microbial diversity in the environment. . Metagenomic is considered as a powerful method to study the bacterial diversity in environmental samples without using any cultural techniques. It provides a broader knowledge of bacterial diversity and avoid any risks. The present research was done on bacterial diversity in water samples from Domestic wastewater. Indian by using Metagenomics, new technology in the world of genetic engineering field. DNA sequences or phylogenetic analysis depended on a single gene, the 16S small subunit ribosomal RNA (rRNA) gene. DNA nucleotide sequence were analyzed with 16s r RNA by BLAST and phylogenetic tree to obtained the relation between types of bacterial sequence get in the present study. The results of this study identified 7 new strains that included 05 families: Vibrio, Streptococcus , pseudomonas, Escherichia coli, Mostly identified bacteria were pathogenic to humans. This study will help in reducing the water borne diseases which are to using several emerging bacterial pathogens. This diversity study proves that most the species identified shows toxic characters.