| dc.description.abstract | Abstract: The current study was designed to isolate and identify of forty-three samples were collected from Mosallata Central Hospital, which included different samples. Including urine, ear, sputum, and nose and wound swabs. The resistance strains increased the challenge in treating the infections caused by S. aureus, which are resistant to antibiotics. The present work aims to Isolate and identifying of S. aureus and detects its resistance and sensitive to common use antibiotics. While the number of isolates from the hospital environmental was five isolates, at a rate of (20.8%), followed by nasal samples, with four isolates with a rate of (16.6%). After that, the wound sample by three isolates at a rate of (12.5%), and at least the ear samples, by two isolates at a rate of (8.3%). Susceptibility test was determined for 43 isolates of S. aureus against 06 different antibiotics penicillin G (10 units), gentamicin (10µg), vancomycin (30µg), cefoxitin (30µg), ampicillin (10µg) and methicillin (5µg), using the disk diffusion method using the Müeller-Hinton agar medium. The growth inhibition zone was measured in millimeters using an accurate ruler in order to read the results and compared to the standard table of the Clinical and Laboratory Standard Institute.
The efficacy of antibiotics against S. aureus which was as follow was 23.2% in penicillin, 13.9% in methicillin, 9.3% in vancomycin, 6.9% in ampicillin, 4.6 in cefoxitin, 2.3% in gentamicin. In the conflicting of that, the other isolates of S. aureus which were resistant to antibiotics appears efficacy against antibiotics as follow was 16.2% in penicillin, 6.9% in both methicillin and vancomycin, 4.6% in both ampicillin and cefoxitin, but no resistant appear against gentamicin. | en_US |
